CRISPR/cas9基因编辑技术用于插入内源性核糖体进入点2序列、FlpO重组酶基因、牛生长激素polyA序列、attB位点、PGK/gb2启动子-诺卡因抗性基因-PGKpolyA插件,以及终止密码子后紧跟的attP位点。引导载体设计使得SpCas9蛋白仅在胚胎干细胞(ES)中表达,且SpCas9DNA序列不整合到基因组中。(来源:J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S6/SvEvTac x C57BL/6NCrl)F1
Endonuclease-mediated
插入
--
1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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