A loxP site was introduced to intron 1, and a loxP-FRT-Neo-FRT-F3 cassette was placed in intron 2 via homologous recombination. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:101977)
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A loxP site was introduced to intron 1, and a loxP-FRT-Neo-FRT-F3 cassette was placed in intron 2 via homologous recombination. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:101977)