Exon 1,包含起始翻译位点的部分,被替换了抗氨苄青霉素抗性基因座。 Western blot 和 qRT-PCR 验证了这个等位基因不产生肽和转录本表达。(来源:J:303193)
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Exon 1, which includes the translation start site, was replaced with a neomycin resistance gene cassette. Western blots and qRT-PCR confirmed the lack of peptide and transcript expression from this allele. (J:303193)