CRISPR/cas9通过重组产生了1号外显子的缺失。使用引物TGAAGCATCCCAAGAAATCC和CACAGTCCTGTCCTGGTGTG,目标区域剩余的序列是GCACACTGCCTCTGAGATGA。在来自纯合小鼠的体外培养成年背根神经节细胞中,表达减少了超过90%(来源:J:268832)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6
Endonuclease-mediated
基因内删除
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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