Silc1^em1Uli

CRISPR/cas9 mediated recombination created a deletion in exon 1. Using the forward primer TGAAGCATCCCAAGAAATCC and the reverse primer CACAGTCCTGTCCTGGTGTG the sequence remaining in the targeted region is GCACACTGCCTCTGAGATGA. Expression is reduced by more than 90% in cultured adult dorsal root ganglion cells from homozygous mice. (J:268832)