CRISPR/Cas9基因编辑技术用于产生了902bp的大规模缺失, Western blot结果显示,纯合突变雄性小鼠的睾丸和附睾精子中没有蛋白质表达。(来源:J:303071)
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CRISPR/Cas9 genome editing technology was used to generate a large 902 bp deletion. Western blot analysis confirmed the absence of protein expression in the testes and cauda epididymal spermatozoa from homozygous mutant male mice. (J:303071)