开放阅读框被替换成了β- gal/neo 嵌入式元件。 Western blot 分析证实了纯合突变小鼠大脑中没有蛋白质表达。(来源:J:302852, J:302897)
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The open reading frame was replaced with a beta-gal/neo cassette. Western blot analysis confirmed the absence of protein expression in the brain of homozygous mutant mice. (J:302852, J:302897)