Tg(CAG-GFP/LC3/RFP/LC3*)2Nmz

This transgene expresses GFP-LC3-RFP-LC3DeltaG, a fluorescent probe to evaluate autophagic flux, under the control of a CAG promoter. The fusion protein is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP-LC3DeltaG. GFP-LC3 is degraded by autophagy, while RFP-LC3DeltaG remains in the cytosol, serving as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. In this line, high expression was observed only in the skeletal muscle. (J:252211)