靶向载体设计为在exon 2上游插入一个loxP位点,随后是exon 5下游带有FRT标签的PGK-neo cassette,由Flp介导的重组会去除FRT标记的neo cassette,使得exons 2到5都被留下了去失框的区域。(来源:J:245204)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count