Rpe65^{tm1.1(cre/ERT2)Kser}

The targeting construct was designed to fuse a P2A self-cleaving peptide sequence and a cre/ERT2 sequence in frame at the 3' of exon 14 of the retinal pigment epithelium 65 (Rpe65) gene. The cre-ERT2 fusion gene contains a Cre recombinase fused to a human estrogen receptor ligand binding domain. A frt-flanked neomycin resistance (neo) cassette was placed immediately after the non-coding exon 15. This construct was electroporated into C57BL/6 FLP-derived iTL BF1 embryonic stem (ES) cells. The Neo cassette in the targeting sequence was removed during ES clone expansion. (J:310003)