Dsg2^{tm1d(EUCOMM)Wtsi}

The L1L2_Bact_P cassette was inserted at position 20711672 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 20712812. The critical exons 4 and 5 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene and subsequent cre expression deleted exons 4 and 5. Exon 4 and 5 deletion causes a frameshift mutation and premature termination of translation. (J:235770)