Mettl3^tm1.1Jhha

KOMP vector PG00144_Z_5_D09 inserted at position 52535930 of Chromosome 14 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 52536652. The critical exon 4 is thus flanked by loxP sites. This "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying the Mettl3tm1Jhha allele. (J:299108)