Exon 3 was targeted with sgRNAs (targeting CTTACTTGAGCCCACAGGCA and ACAGAACCATTTTGTACTAG) using CRISPR/Cas9 technology, resulting in a 44 bp deletion. This allele occurs in conjunction with the HREF="http://www.informatics.jax.org/allele/MGI:6491613">Ythdf1em1Jhha, Ythdf1em3Jhha and Ythdf3em1Jhha alleles in the same ESC line. (J:299108)