通过sgRNAs(针对CGGGAGCAGAAAGGCAAG)和ssODN模板(序列CGGTTTGTCTAATTAAACCATAATATCCTGCTTCCTTACAGCGTACAGGCAGGAACAGAAGGGCAAGAAGGAGGCTTCGGAGCAAAAGAAGAAGGACAAAGACAAAAAGGA)使用CRISPR/Cas9技术,产生了一个2个碱基对的插入,插入位置在编码区域的3'末端,可能是GC或CG。这个插入导致了编码肽正常带有负电荷的尾部形成一个大的净正电荷。(来源:J:298827)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
FVB
Endonuclease-mediated
插入
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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