The 8.4kb construct incorporates the mouse Col2a1 promoter and a fragment of intron 1 ligated to a splice acceptor sequence followed by an internal ribosomeentry site (IRES), tetracyclinecontrolled transactivator (tTA), and the SV40 large T antigen polyadenylation signal. A single copy of the transgene was inserted 16 kb downstream of Peli2 on mouse Chromosome 14. Line H is the high expresser line. (J:320594)