在exon 9的3' UTR末尾,通过CRISPR/Cas9技术插入了包含三个FLAG标签、一个HA标签、p2A自剪切序列以及GFP荧光标记基因的序列。gRNAs为GCCTCAGGTCACATTATCGG(tgg)和TGCAATCAGTTCCGACTCTC(tgg)。随后,插入了一段抗氨苄青霉素的耐药基因元件。(来源:J:290712)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6J x 129S6/SvEvTac)F1
Endonuclease-mediated
插入
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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