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A loxP-flanked transcriptional blocking cassette was inserted at a site 11 base pairs upstream of the translational start site of the gene in exon 2 using BAC recombineering techniques generating a null allele. The transcriptional blocking cassette includes a splice acceptor site from the mouse engrailed 2 gene, an SV40 poly(A), an SV40 enhancer, a neomycin resistance gene, two HSV-TK poly(A) signals, a synthetic poly(A) signal/transcriptional pause signal, another synthetic poly(A) signal, and a Myc-associated zinc finger protein-binding site. (J:298337)