Trp63^tm3.2Aam

The codon for arginine 318 (AGA) was changed to the codon for histidine (CAT) in exon 7 (R318H) which is equivalent to the R279H mutation in human ectrodactyly, ectodermal dysplasia, clefting syndrome. In addition, a neomycin cassette flanked by loxP sites was inserted upstream of exon 5 and a loxP site was inserted downstream of exon 7. The neomycin cassette and exons 5 to 7 encoding the DNA binding domain were removed via cre-mediated recombination generating a null allele. (J:294158)