Exon 1用sgRNAs( TAGGCTCTCTGCAATGGCTGAC, AAACGTCAGCCATTGCAGAGAG, TAGGGGTAAGCAGGTTGCTGA和AAACCTCAGCAACCTGCTTACC)进行靶向,通过CRISPR/Cas9技术,与一个包含GFP基因和poly(A)信号序列的捐赠质粒一起构建。这产生了缺失报告基因,其中Exon 1被GFP基因替换。(来源:J:293325)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count