Exon 1用sgRNAs( TAGGCTCTCTGCAATGGCTGAC, AAACGTCAGCCATTGCAGAGAG, TAGGGGTAAGCAGGTTGCTGA和AAACCTCAGCAACCTGCTTACC)进行靶向,通过CRISPR/Cas9技术,与一个包含GFP基因和poly(A)信号序列的捐赠质粒一起构建。这产生了缺失报告基因,其中Exon 1被GFP基因替换。(来源:J:293325)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6
Endonuclease-mediated
插入,基因内删除
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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