Exon 1通过两个TALENs(序列GCACCATGCCTGCCTG和AGTAGGAGGCCAGCGGC)进行了靶向,产生了17个bp的缺失(序列TCGCCTCTGCTTGCTGG)。qRT-PCR和 Western blot的结果表明,这个等位基因没有检测到mRNA和蛋白质的表达。(来源:J:293623)
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Exon 1 was targeted with two TALENs (GCACCATGCCTGCCTG and AGTAGGAGGCCAGCGGC), resulting in a 17 bp deletion (TCGCCTCTGCTTGCTGG). qRT-PCR and Western blots showed absence of mRNA and protein expression from this allele. (J:293623)