在exon 8的编码序列末尾插入了CRISPR靶向,连接了一个GSG(甘氨酸-丝氨酸-甘氨酸)的链接器,猪细小病毒-1的2A( P2A)多肽,SV40病毒的NLS,P1样转导噬菌体D6的重组酶基因,以及由FRT序列 flank的 puromycin抗性基因座。通过Flp介导的重组,去除了选择性基因座。(来源:J:284134)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6NCrlCrlj
Endonuclease-mediated
插入
--
1
3
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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