整个编码区域被替换成lacZ-loxP-U-b1-em7-neo-loxP片段。RT-PCR和 Western blot 检测证实了纯合小鼠中无表达。(来源:J:294591)
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The entire coding region was replaced with a lacZ-loxP-U-b1-em7-neo-loxP cassette. RT-PCR and western blot analysis confirmed the absence of expression in homozygous mice. (J:294591)