在第15外显子的磷酸化接受位点Ser468和Ser485被替换为Alanine,分别标记为S468A和S485A。正常基因的第15外显子,通过loxP位点被克隆,插入在第14-15内含子上游,紧接着是转录终止信号。接着,一个FRT引导的诺卡因选择性 cassette被插入到第14-15内含子。通过FlpE介导的重组,移除了neo cassette。(来源:J:296139)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
Insertion, Nucleotide substitutions
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
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