这个靶向载体包含一个5.5kb的5'臂和3.5kb的3'臂,分别插入了Lox71和Lox2272的序列,这些位置是为了在基因的周围。基因的cDNA被替换为编码带有HA标签的Ngn3蛋白的序列。载体中还包含一个FRT引导的Pgk-Puro-deltaTK元件,用于通过puromycin进行阳性选择(针对靶向事件)以及通过ganciclovir进行阴性选择(针对RMCE事件)。来源:(J:296396)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S6/SvEvTac
Targeted
插入,基因内删除
--
1
1
--

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部