Rtp4^em#Xzs

The first and second coding exons (1 and 2) were targeted with two sgRNAs (5-ccccgatgacttcagtacttggg-3 and 5- cctcagacccggaatacggatttt-3) using CRISPR/Cas9 technology. Five founder mice were created with at least two different size deletions, both including the end of exon 1, intron 1 and the start of exon 2. The pound # sign is used in the allele symbol because which mouse line was used, or if a mixture was used, is not specified. (J:293929)