Kras^tm1Kshn

A loxP site flanked STOP cassette, with a 5' puromycin resistance gene cassette, was inserted into intron 2. Endogenous exon 3 was modified to harbor a C-to-T mutation at codon 58, resulting in a threonine to isoleucine mutation (p.T58I) associated with patients with Noonan syndrome (NS). (J:296368)