Nras^tm1.1Kshn

The targeting vector is designed to insert an FRT-flanked neomycin resistance (neo) cassette downstream of exon 5. Endogenous exon 5 was modified to harbor a guanine to cytosine mutation at codon 181, resulting in a cysteine to serine mutation (C181S). This targeting vector then was used to retarget Nrastm1Tyj inserting C181S in cis to the existing G12Dmutation. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:289386)