靶向载体被设计成在mRNA的5' UTR上游插入一个loxP位点,紧邻第一个编码外显子(exon 2)。随后在3' UTR下游插入第二个loxP位点,接着是一个由FRT引导的PGK-neo cassette,后面跟着第三个loxP位点。通过Flp介导的重组,FRT引导的neo cassette被移除,使得exon 2区域被标记为可剪接。(来源:J:287983)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Targeted
插入
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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