通过使用BAC克隆RP23-192F6,研究人员在内含子1位置插入了loxP位点,并在内含子3处添加了FRT序列,伴随着抗氨苄青霉素的耐药基因座。随后通过flp介导的重组,移除了neo cassette,从而得到了一个前体条件性基因,它有两个外显子2和3被去除了(来源:J:294178)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129/Sv x C57BL/6J)F1
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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