4930451I11Rik^em2Osb

CRISPR/cas9 mediated recombination created a 246 bp deletion around exon 3, including 88bp from exon 3 and 12bp from exon 4 resulting in an in-frame mutation and premature stop codon at the 85th amino acid. This is predicted to disrupt only the transmembrane isoform. RT-PCR analysis confirmed the absence of the transmembrane form and presence of the secreted form. (J:287619)