使用CRISPR/Cas9技术,通过一个针对多个基因簇成员的特异性强的sgRNA,以下基因簇成员的部分或全部被删除:Klra11-ps(5'端),Klra8和Klra14-ps(3'端)(大约66kb的删除);Klra7(5'端),Klra10,Klra13-ps,Klra3以及Klra1(3'端)(大约149kb的删除)。流式细胞术确认了编码蛋白的基因丧失表达。未被删除的Klra4的表达显著降低,这表明删除区域包含调控序列。来源:(J:295190)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6
Endonuclease-mediated
Intergenic deletion, Intragenic deletion
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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