Cfap43^em1Sono

CRISPR/Cas9 technology generated a 14 bp deletion in exon 35 resulting in a premature stop codon (p.Asn1532GlysfsTer36) and which is predicted to produce a truncated nonfunctional protein. RT-PCR confirmed the 14 bp deletion. Quantitative RT-PCR detects very low expression in the brain and trachea of homozygotes. (J:285551)