CRISPR技术替换了1到3号外显子,用了一个诺卡因抗性基因座。 Western blot结果显示肺部没有检测到蛋白表达。(来源:J:288329)
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CRISPR-targeting replaced exons 1 through 3 with a neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in the lungs. (J:288329)