通过CRISPR技术删除了53bp,产生了框架移位和提前终止, Western blot结果显示多种组织中没有检测到蛋白质表达。(来源:J:289414)
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CRISPR-targeting deleted 53 bp to create a frameshift and premature termination. Western blot analysis confirmed the absence of protein expression in several tissues. (J:289414)