Tmprss12^em4Osb

CRISPR-targeting of exon 2 deleted 8 bp within the intron with the indel -7871 bp, +5 bp between intron 2 and exon 5 to produce a frameshift mutation with a premature stop codon at the 70th amino acid. Western blot analysis confirmed the absence of protein expression in testicular giant cells and spermatozoa. (J:293874)