Lipa^{tm1d(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 34488106 of Chromosome 19 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 34489566. Exon 4 is thus flanked by loxP sites. The lacZ and neo cassettes were removed through subsequent flp-mediated recombination and exon 4 was deleted through cre-mediated recombination after that, creating a null allele. (J:294097)