Two successive stop codons were inserted in exon 32 of the gene. The mutations introduced were c.6052C > T (p.Arg2018*), corresponding to c.6091C > T (p.Arg2031*) in human and c.6061C > T (p.Gln2021*), corresponding to c.6100C > T (p.Arg2034*) in human. An adjacent frt-flanked neomycin resistance cassette was removed via FLP-mediated recombination. (J:274090)