Ryr1^tm2.1Mfgo

The targeting vector is designed to insert an FRT-flanked neomycin resistance gene upstream of exon 34, an amino acid substitution at position 1669 altering serine to cysteine (S1669C; TCG to TGC) and a Leu1716 deletion (CTC) into exon 34. A silent mutation Leu1715 (CTC to CTG) was introduced to create a Bcl1 enzyme site. The neo cassette was deleted by transient transfection of an expression vector of flp recombinase in the ES cells. (J:101977)