在终止密码子上游的目标序列,通过CRISPR/Cas9技术用gRNA(5'-GGGACCTACAGCAGAATAG-3')和 oligonucleotide 进行了HA标签序列的插入。生成的等位基因具有九个C末端HA氨基酸,它们在终止密码子正上游成框架内插入。(来源:J:291761)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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