Sh2b1^em1Ccsu

CRISPR/cas9 endonuclease-mediated genome editing was used to create an amino acid substitution at position 322 altering proline to serine (P322S; CCC to TCC) in exon 3 of the gene. The mutation is located in the pleckstrin domain (PH), and was identified as a human childhood obesity-associated variant allele. (J:280476)