Tg(tetO-Btnl1)#Ahay

The cDNA for exons 2-9 and the coding part of exon 10, comprising the entire coding region plus part of the 5' UTR, was placed downstream of a tetracycline response element (TRE) and minimal CMV promoter, followed by beta-globin intron and poly signal sequences. Expression of this transgene can be controlled by tetracycline/doxycycline in conjunction with a tTA or rtTA expressing allele. The transgene construct was injected into blastocysts homozygous for the Phenotypes