在终止密码子之前插入了一个荧光素/羟氨霉素抗性融合元件。在去雄性大脑组织的 Western 印迹中未检测到 MECP2 蛋白。(来源:J:222245)
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A luciferase/hygromycin resistance fusion cassette was introduced in frame immediately before the termination codon. No MECP2 protein is detectable in Western blots of hemizygous male brain tissue. (J:222245)