Ncoa1^tm1.1So

The targeting construct is designed to insert a loxP site downstream of exon 14 and an FRT-flanked neomycin (neo) resistance cassette, followed by a second loxP site downstream of exon 16. A CCA to TCA mutation was introduced in exon 16, encoding activation domain 2, converting a proline to a serine (P1278S) similar to a mutation found in a small cohort of bone metastases from breast cancer patients. Flp-mediated recombination removed the FRT-flanked neomycin cassette. (J:341575)