Igs7^{tm166(tetO-EGFP*,CAG-tTA2)Hze}

The reporter contains a cre-dependent, tetracycline-activated tTA2-TRE transcriptional cassette combined with a Kozak consensus start site (for strong initiation of translation), two Myc-tags, an optimized G)22(-repeat MORF translation switch, and a farnesylated EGFP fluorescent reporter. The farnesylation sequence (CAAX domain from Ras) targets the reporter to membranous structures including neuronal dendrites and axons. Specifically, the final vector contains from 5' to 3': FRT2, 2x Ins, TRE promoter, loxP-flanked STOP sequence, a Kozak consensus start site (for strong initiation of translation),two Myc-tags, an optimized G)22(-repeat MORF switch, a farnesylated EGFP fluorescent reporter (EGFP-F), WPRE, pA, 2X Ins, CAG promoter, lox2272-flanked STOP sequence, tTA2, WPRE, pA and an attB-PGK-5';hygro-SD-frt5-SA 3';hygro-SV40pA-attPan FRT site, inserted into the T1 TIGRE locus. (J:292704)