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设计的质粒含有一种导向RNA，用于在第17外显子插入D801N突变。CRISPR/Cas9导向策略中额外加入了一个无意义的核苷酸变化，从CTG变为CTA（L802L），这个改变不影响蛋白质序列。通过基因测序和PCR，我们确认了目标精确的幼崽。(来源：J:94077)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
