CRISPR/Cas9基因编辑技术用于将终止密码子替换为含有P2A肽序列和荧光报告基因的双顺式报告载体,后者带有阿米替林荧光标记。此外,基因的3'端附近发现了一个9个核苷酸的内切缺失,但不认为这会影响蛋白表达。阿米替林是一种源于阿库雷乌斯 victoria 的长余辉黄色荧光蛋白(Excitation at 406 nm, Emission at 526 nm,J:259930)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Endonuclease-mediated
插入,基因内删除
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1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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