CRIPSR技术替换了Mecp2基因(NCBI NM_010788,编码区域为c. 280-492和内含子3,p. 94-164)的甲基-CpG结合域(MBD),使用了MBD2的同源序列(NCBI NM_010773,c. 457-651,不包括内含子,p. 153-217)。基因的C端通过连接了EGFP和一个短连接肽(TGTAAGGATCCACCGGTCGCCACC)进行标记。随后,Cre介导的重组去除了内含子2中的选择性 cassette。来源:J:309631

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Endonuclease-mediated
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1
15
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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