CRISPR/Cas9技术产生了一个316bp的缺失,包括了整个exon3,导致了框架移位。 Western blot结果显示TESMIN-L蛋白缺失,而RT-PCR证实了Tesmin-S转录本的不存在。(来源:J:288964)
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CRISPR/Cas9 technology generated a 316 bp deletion that includes the entire exon 3 resulting in a frameshift. Western blot analysis confirmed the absence of TESMIN-L protein and RT-PCR confirmed the absence of Tesmin-S transcript. (J:288964)