CRISPR/Cas9技术将全长的人ACE2基因、IRES和tdTomato基因插入了小鼠基因的第2外显子。 Western blot结果显示,人类基因在肺和肾中表达强烈,而在脾和肝中的表达则很弱,与小鼠蛋白的表达模式相似。PCR实验确认了小鼠基因的缺失。(来源:J:288243)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Endonuclease-mediated
插入
--
1
--
8

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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