Ace2^{em1(ACE2)Yowa}

CRISPR/Cas9 technology inserted the full-length human ACE2 cDNA, IRES, and tdTomato gene into exon 2 of the mouse gene. Western blot analysis confirmed strong expression of the human gene in lung and kidney and very weak expression in spleen and liver, a similar expression patter to the mouse protein. PCR confirmed the absence of the mouse gene. (J:288243)