Gt(ROSA)26Sor^{tm1(tTA,tetO-Mtor*D2338A,-IRES-EGFP)Ebm}

The transgenic construct inserted into the GT(ROSA)26Sor locus contains a splice-acceptor sequence, loxP-flanked neomycin selection cassette, a tetracycline transactivator (tTA) gene, insulator sequence, CMV promoter responsive to tTA, rabbit -globin intron, Mtor cDNA encoding a kinase dead protein, an internal ribosome entry site, and an enhanced green fluorescence protein (EGFP) cDNA. Expression of the tetracycline transactivator is turned on upon Cre-mediated recombination, and removal of the neomycin selection cassette induces Mtor expression in the absence of doxycycline. (J:252107)