Bola2^{tm1.1(KOMP)Wtsi}

The insertion of the L1L2_Bact_P cassette created a deletion of size 564 starting at position 126295347 and ending at position 126295911 of Chromosome 7 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. FLP-mediated recombination removed the lacZ and neomycin selectino cassette in the germline. (J:284746)